Further indications, specificity and sensitivity, method of detection, sample material, sample stability and shipment, short protocol
- Complementary investigation in the diagnosis of renal cell carcinoma
- Monitoring response to therapy
- Early detection of relapse or of metastasis
- Monitoring of tumor agressiveness
- Diagnostic support and patient follow-up:
– Colorectal Cancer
– Gastric Cancer
– Oesophageal Carcinoma
– Lung Cancer
– Breast Cancer
– Pancreatic Cancer
High specificity and sensitivity
Renal Cell Carcinoma:
Specificity: >90% Sensitivity: 70-100% (depends on Robson stage)
Methode of detection
- Human EDTA plasma only.
- Blood collection and centrifugation must be done on the same day.
- EDTA plasma samples can be stored at -20°C for up to one year.
Sample stability and shipment
- Only EDTA plasma samples should be used.
- 12 hours at room temperature (only suitable for dispatch within a hospital or diagnostic center or by courier).
- Up to 3 days at 4° – 8°C.
- Up to 1 year at -20°C.
Short protocol for the experienced user
Important: The short protocol is not a substitute for the detailed protocol given in the instruction manual!
- Prepare the sample-/washing buffer
- Dilute EDTA-plasma samples 1:100 with sample-/washing buffer
- Pipette 50 µl blank, standards (ready-to-use), control (ready-to-use) and samples in duplicate into the ELISA-strips
- Incubate 60 minutes at room temperature
- 50 µl anti M2-PK bio (1:100)
- Incubate 30 minutes at room temperature
- 50 µl POD-Streptavidin (ready-to-use)
- Incubate 30 minutes at room temperature (in the dark)
- 100 µl substrate solution (ready-to-use)
- Incubate 15 minutes at room temperature (in the dark)
- Add 100 µl stop solution (ready-to-use)
- Read plate at OD 450 or OD 450 – OD 620
- Evaluate with standard curve using a log-log scale