The metabolic biomarker “Faecal M2-PK” is a screening parameter for colorectal (bowel) cancer and polyps and is independent of “Faecal Occult Blood” (FOB).

Tumor-M2-PK-Stool-TestThe ScheBo®  Tumor M2-PK™ Stool Test is a totally new approach for bowel cancer secreening tests. Previosly, only non-specific tests for blood in the stool could be used to give an indication of an existing bowel cancer or its precursors. With the new ELISA method for M2-PK in the stool it′s now possible to detect bleeding or non-bleeding bowel cancers, as well as polyps. The test is not dependent on occult blood.

Advantages, method of detection, sample material, short protocol


The main advantages of the ScheBo® Tumor M2-PK™ Stool Test are:

  • High sensitivity
  • High specificity
  • Not dependend on occult blood
  • Detects bleeding or non-bleeding bowel polyps, especially >1cm
  • Detects bleeding or non-bleeding bowel tumours
  • Not affected by particular foods
  • No special diet required
  • No false results from haemorrhoids or other sources of blood in the bowel
  • A single pea-sized sample is sufficient

Methode of detection

Sandwich ELISA with two monoclonal antibodies highly specific for human M2-PK. The ELISA kit is based on a microtiter plate (96 well format) with 12 single strips x 8 wells suitable for up to 42 samples in duplicate.

Sample material

A single pea-sized stool sample is sufficient.

Short protocol for the experienced user

ScheBo®  Tumor M2-PK™ Stool Test for the quantitative determination of M2-PK by healthcare professionals.

Important: The short protocol is not a substitute for the detailed protocol given in the instruction manual!

  • Prepare the washing buffer (and the extraction buffer)
  • Extract stool and homogenize
  • Reconstitute lyophilized standards and controls with washing buffer
  • Pipette 50 µl blank, standards, control and samples in duplicate into the ELISA strips – Incubate 60 minutes at room temperature – Wash
  • 50 µl anti-Tumor M2-PK-biotin and POD-Streptavidin-Complex (ready-to-use) – Incubate 30 minutes at room temperature (in the dark) – Wash
  • 100 µl substrate solution (ready-to-use) – Incubate 15 minutes at room temperature (in the dark)
  • Add 100 µl stop solution (ready-to-use)
  • Read plate at OD 450 or OD 450 – OD 620
  • Evaluate with standard curve using a log-log scale