The test

Indications

• Complementary investigation in the diagnosis of renal cell carcinoma
• Monitoring response to therapy
• Early detection of relapse or of metastasis
• Monitoring of tumor agressiveness
• Diagnostic support and patient follow-up:
  * Colorectal Cancer
  * Gastric Cancer
  * Oesophageal Carcinoma
  * Lung Cancer
  * Breast Cancer
  * Pancreatic Cancer

High specificity and sensitivity

Methode of detection

Sample material

• Human EDTA plasma only.
• Blood collection and centrifugation must be done on the same day.
• EDTA plasma samples can be stored at -20°C for up to one year.

Sample stability and shipment

• Only EDTA plasma samples should be used.
• 12 hours at room temperature (only suitable for dispatch within a hospital or diagnostic center or by courier).
• Up to 3 days at 4° - 8°C.
• Up to 1 year at -20°C.

Short protocol for the experienced user

• Prepare the sample-/washing buffer
• Dilute EDTA-plasma samples 1:100 with sample-/washing buffer
• Pipette 50 µl blank, standards (ready-to-use), control (ready-to-use) and samples in duplicate into the ELISA-strips
• Incubate 60 minutes at room temperature
• Wash
• 50 µl anti Tumor M2-PK bio (1:100)
• Incubate 30 minutes at room temperature
• Wash
• 50 µl POD-Streptavidin (ready-to-use)
• Incubate 30 minutes at room temperature (in the dark)
• Wash
• 100 µl substrate solution (ready-to-use)
• Incubate 15 minutes at room temperature (in the dark)
• Add 100 µl stop solution (ready-to-use)
• Read plate at OD 450 or OD 450 - OD 620
• Evaluate with standard curve using a log-log scale